Quinn’s advantage fertilization medium enhances zona pellucida-induced acrosome reaction compared with human tubal fluid medium
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چکیده
منابع مشابه
Acrosome reaction: relevance of zona pellucida glycoproteins.
During mammalian fertilisation, the zona pellucida (ZP) matrix surrounding the oocyte is responsible for the binding of the spermatozoa to the oocyte and induction of the acrosome reaction (AR) in the ZP-bound spermatozoon. The AR is crucial for the penetration of the ZP matrix by spermatozoa. The ZP matrix in mice is composed of three glycoproteins designated ZP1, ZP2 and ZP3, whereas in human...
متن کاملHyperactivation of capacitated human sperm correlates with the zona pellucida-induced acrosome reaction of zona pellucida-bound sperm.
BACKGROUND The aim of this study was to determine the relationship between human sperm hyperactivation (HA), sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction (AR) of ZP-bound sperm in vitro. METHODS Sperm samples from 129 infertile men were studied. Motile sperm (2 x 10(6)) selected by Pure sperm were incubated with four oocytes in 1 ml human tubal fluid supplemented wit...
متن کاملZona pellucida and progesterone-induced Ca2+ signaling and acrosome reaction in human spermatozoa.
The mammalian acrosome reaction (AR) is the fusion, at multiple points, of the outer acrosomal membrane with the overlying plasma membrane. This fusion causes the release of acrosomal contents and the exposure of the inner acrosomal membrane. The time taken for this process to occur is not accurately known. Estimates for AR in mouse spermatozoa vary from ;2 minutes after stimulation with solubi...
متن کاملInteraction of human spermatozoa with the zona pellucida of oocyte: development of the acrosome reaction.
Mammalian spermatozoa are not able to fertilize an oocyte upon ejaculation. To gain fertilizing ability, spermatozoa must, either in vivo or in vitro, undergo a process termed capacitation. Since a reliable marker for capacitation does not exist, it is considered that this process is completed when the spermatozoa are able to undergo acrosomal x intestinal mucosa which might require tandem sign...
متن کاملRecombinant human zona pellucida glycoprotein 3 induces calcium influx and acrosome reaction in human spermatozoa.
Recombinant human ZP3 (rhuZP3) generated by Chinese hamster ovary cells transfected with a plasmid containing human ZP3 cDNA was used to study the acrosome reaction (AR) and intracellular calcium fluxes in capacitated human spermatozoa. Conditioned medium containing rhuZP3 significantly induced the AR (P < or = 0.005) in 59.4 +/- 4.7% of spermatozoa (control = 8.5 +/- 3.1%) and caused complete ...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2011
ISSN: 1472-6483
DOI: 10.1016/j.rbmo.2011.07.013